Regulatory

Part:BBa_K3030008:Design

Designed by: Xiaomeng Liu   Group: iGEM19_XJTLU-CHINA   (2019-09-13)


MS2-responsive ribozyme switch (Off to On)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The location of the sequence, usually on the 3' end of the mRNA, after the coding sequence.


Source

This part is initially designed through physically and functionally combing the sTRSV HHRz actuator and MS2 coat protein aptamer sensor domains. This sequence of the part is from the supplementary data of https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4231745/


References